解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), although the live birth rate is relatively low. Recent studies have identified H3K9me3 in donor cells and abnormal Xist activation as epigenetic barriers that impede SCNT. Here we overcome these barriers using a combination of Xist knockout d...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.06.008
更新日期:2018-09-06 00:00:00
abstract::Reconstructing lineage relationships between cells within a tissue or organism is a long-standing aim in biology. Traditionally, lineage tracing has been achieved through the (genetic) labeling of a cell followed by the tracking of its offspring. Currently, lineage trajectories can also be predicted using single-cell ...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2018.04.014
更新日期:2018-08-02 00:00:00
abstract::Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.06.013
更新日期:2018-08-02 00:00:00
abstract::Alexander disease (AxD) is a leukodystrophy that primarily affects astrocytes and is caused by mutations in the astrocytic filament gene GFAP. While astrocytes are thought to have important roles in controlling myelination, AxD animal models do not recapitulate critical myelination phenotypes and it is therefore not c...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.07.009
更新日期:2018-08-02 00:00:00
abstract::In this issue of Cell Stem Cell, Pei et al. (2018) demonstrate that leukemic stem cells (LSCs) have enhanced mitochondrial fission, which is positively regulated by FIS1. FIS1 is necessary to maintain LSC function; thus, targeting its expression and mitochondrial fission is a novel approach to decrease stemness and in...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.05.026
更新日期:2018-07-05 00:00:00
abstract::Chronic liver injury can cause cirrhosis and impaired liver regeneration, impairing organ function. Adult livers can regenerate in response to parenchymal insults, and multiple cellular sources have been reported to contribute to this response. In this study, we modeled human chronic liver injuries, in which such resp...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.05.022
更新日期:2018-07-05 00:00:00
abstract::Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.04.022
更新日期:2018-06-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) sustain hematopoiesis throughout life. HSCs exit dormancy to restore hemostasis in response to stressful events, such as acute blood loss, and must return to a quiescent state to prevent their exhaustion and resulting bone marrow failure. HSC activation is driven in part through the pho...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.05.003
更新日期:2018-06-01 00:00:00
abstract::The California Institute for Regenerative Medicine (CIRM) Alpha Stem Cell Clinic (ASCC) Network was launched in 2015 to address a compelling unmet medical need for rigorous, FDA-regulated, stem cell-related clinical trials for patients with challenging, incurable diseases. Here, we describe our multi-center experience...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.05.007
更新日期:2018-06-01 00:00:00
abstract::Despite widespread interest in using human induced pluripotent stem cells (hiPSCs) in neurological disease modeling, a suitable model system to study human neuronal connectivity is lacking. Here, we report a comprehensive and efficient differentiation paradigm for hiPSCs that generate multiple CA3 pyramidal neuron sub...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.04.009
更新日期:2018-05-03 00:00:00
abstract::While gene expression dynamics have been extensively cataloged during hematopoietic differentiation in the adult, less is known about transcriptome diversity of human hematopoietic stem cells (HSCs) during development. To characterize transcriptional and post-transcriptional changes in HSCs during development, we leve...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2018.03.012
更新日期:2018-04-05 00:00:00
abstract::DNA methylation is an epigenetic mark that plays pivotal roles in gene regulation, but its functions in neural fate decisions are poorly understood. In this issue of Cell Stem Cell, Ziller et al. (2018) show that the de novo methyltransferase Dnmt3a ensures efficient generation of motor neurons from stem cells. ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.03.006
更新日期:2018-04-05 00:00:00
abstract::The emerging field of regenerative rehabilitation integrates biological and bioengineering advances in regenerative medicine with rehabilitative sciences. Here we highlight recent stem cell-based examples of the regenerative rehabilitation paradigm to promote tissue repair and regeneration, and we discuss remaining ch...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2018.02.003
更新日期:2018-03-01 00:00:00
abstract::Muscle stem cell regenerative capacity is rapidly lost during ex vivo culture. In this issue of Cell Stem Cell, Judson et al. (2018) show that inhibition of cytoplasmic SETD7, a lysine methyltransferase, potently expands naive, undifferentiated mouse and human muscle stem cells by restricting their progression through...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2018.01.007
更新日期:2018-02-01 00:00:00
abstract::Minority subpopulations within embryonic stem cell cultures display an expanded developmental potential similar to that of early embryo blastomeres or the early inner cell mass. The ability to isolate and culture totipotent cells capable of giving rise to the entire conceptus would enhance our capacity to study early ...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2017.12.011
更新日期:2018-01-04 00:00:00
abstract::Precise lineage trajectories and the cellular sources that contribute to regeneration after injury are largely unknown in many tissues. In this issue of Cell Stem Cell, Gadye et al. (2017) and Lin et al. (2017) show that olfactory epithelial cells transit through unique and unfamiliar paths of differentiation and unde...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.11.014
更新日期:2017-12-07 00:00:00
abstract::Myeloid-biased hematopoietic stem cells (MB-HSCs) play critical roles in recovery from injury, but little is known about how they are regulated within the bone marrow niche. Here we describe an auto-/paracrine physiologic circuit that controls quiescence of MB-HSCs and hematopoietic progenitors marked by histidine dec...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.11.003
更新日期:2017-12-07 00:00:00
abstract::Stem cell-based therapies for Parkinson's disease are moving into a new and exciting era, with several groups pursuing clinical trials with pluripotent stem cell (PSC)-derived dopamine neurons. As many groups have ongoing or completed GMP-level cell manufacturing, we highlight key clinical translation considerations f...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.09.014
更新日期:2017-11-02 00:00:00
abstract::Intestinal organoids offer great promise for modeling intestinal diseases; however, harvesting intestinal tissue is invasive and directed hPSC differentiation protocols are laborious and costly. In this issue of Cell Stem Cell, Miura and Suzuki (2017) describe the direct conversion of somatic cells from both mice and ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.09.011
更新日期:2017-10-05 00:00:00
abstract::Organoid techniques provide unique platforms to model brain development and neurological disorders. Whereas several methods for recapitulating corticogenesis have been described, a system modeling human medial ganglionic eminence (MGE) development, a critical ventral brain domain producing cortical interneurons and re...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.07.007
更新日期:2017-09-07 00:00:00
abstract::Most disease variants lie within noncoding genomic regions, making their functional interpretation challenging. Because chromatin openness strongly influences transcriptional activity, we hypothesized that cell-type-specific open chromatin regions (OCRs) might highlight disease-relevant noncoding sequences. To investi...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.07.008
更新日期:2017-09-07 00:00:00
abstract::3D organoids enable in vitro human brain development models, but they have not yet recapitulated some essential features of brain circuit formation. Recently, several studies appearing in Nature, Nature Methods, and Cell Stem Cell generated fused organoid models of inhibitory and excitatory neuron development, which c...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.08.017
更新日期:2017-09-07 00:00:00
abstract::Cerebral organoids represent a new model in which to study human brain development outside the human body. Recently in Nature Biotechnology, Lancaster et al. (2017) tackled the lack of reproducibility, tissue homogeneity, and complexity of this system by bioengineering organoids to establish the next generation of hum...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.07.001
更新日期:2017-08-03 00:00:00
abstract::Several cell populations have been reported to possess intestinal stem cell (ISC) activity during homeostasis and injury-induced regeneration. Here, we explored inter-relationships between putative mouse ISC populations by comparative RNA-sequencing (RNA-seq). The transcriptomes of multiple cycling ISC populations clo...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.06.014
更新日期:2017-07-06 00:00:00
abstract::Organ growth requires a careful balance between stem cell self-renewal and lineage commitment to ensure proper tissue expansion. The cellular and molecular mechanisms that mediate this balance are unresolved in most organs, including skeletal muscle. Here we identify a long-lived stem cell pool that mediates growth of...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.06.003
更新日期:2017-07-06 00:00:00
abstract::Functionally relevant markers of glioblastoma stem-like cells (GSCs) have potential for therapeutic targeting to treat this aggressive disease. Here we used generation and screening of thousands of monoclonal antibodies to search for receptors and signaling pathways preferentially enriched in GSCs. We identified integ...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.04.009
更新日期:2017-07-06 00:00:00
abstract::Over the past decade, our growing understanding of cancer stem cell characteristics and function continues to guide innovative new treatments to eradicate cancer. For our tenth anniversary, we asked authors who cited a selection of our most popular papers in this area about the broad influences these studies have had....
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.05.005
更新日期:2017-06-01 00:00:00
abstract::Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche cells in female and ma...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.03.007
更新日期:2017-06-01 00:00:00
abstract::Human disease phenotypes associated with haploinsufficient gene requirements are often not recapitulated well in animal models. Here, we have investigated the association between human GATA6 haploinsufficiency and a wide range of clinical phenotypes that include neonatal and adult-onset diabetes using CRISPR (clustere...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.01.001
更新日期:2017-05-04 00:00:00
abstract::Mammalian embryonic stem cells (ESCs) can self-organize in vitro, but whether they can recreate early embryonic morphogenesis is unclear. Harrison et al. recently demonstrate in Science that 3D co-cultures of mouse ESCs and trophoblast stem cells self-organize into embryo-like structures that recreate many features of...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.04.001
更新日期:2017-05-04 00:00:00
abstract::In this study, we used whole-genome sequencing and gene expression profiling of 215 human induced pluripotent stem cell (iPSC) lines from different donors to identify genetic variants associated with RNA expression for 5,746 genes. We were able to predict causal variants for these expression quantitative trait loci (e...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2017.03.009
更新日期:2017-04-06 00:00:00
abstract::RNA-binding proteins (RBPs), in addition to their functions in cellular homeostasis, play important roles in lineage specification and maintaining cellular identity. Despite their diverse and essential functions, which touch on nearly all aspects of RNA metabolism, the roles of RBPs in somatic cell reprogramming are p...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.12.002
更新日期:2017-04-06 00:00:00
abstract::Tumors contain hostile inflammatory signals generated by aberrant proliferation, necrosis, and hypoxia. These signals are sensed and acted upon acutely by the Toll-like receptors (TLRs) to halt proliferation and activate an immune response. Despite the presence of TLR ligands within the microenvironment, tumors progre...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.12.001
更新日期:2017-04-06 00:00:00
abstract::Prognostic markers that capture leukemia stem cell (LSC) activity can be useful for the risk stratification of acute myeloid leukemia (AML) patients. In a recent issue of Nature, Ng et al. (2016) develop a prognostic score based on a 17-gene expression signature of LSCs to predict outcome in AML patients. ...
journal_title:Cell stem cell
pub_type: 评论,杂志文章
doi:10.1016/j.stem.2017.02.010
更新日期:2017-03-02 00:00:00
abstract::Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enuc...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.10.001
更新日期:2017-01-05 00:00:00
abstract::New methods to manipulate gene and cell state can be used to engineer cell functionality, simplify quality assessment, and enhance manufacturability. These strategies could help overcome unresolved cell therapy manufacturing challenges and complement frameworks to design quality into these complex cellular systems, ul...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.12.003
更新日期:2017-01-05 00:00:00
abstract::Naive human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts, but their X chromosome state has remained unresolved. Here, we show that the inactive X chromosome (Xi) of primed hESCs was reactivated in naive culture conditions. Like cells of the blastocyst, the resulting naive ...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.10.006
更新日期:2017-01-05 00:00:00
abstract::The generation of distinct hematopoietic cell types, including tissue-resident immune cells, distinguishes fetal from adult hematopoiesis. However, the mechanisms underlying differential cell production to generate a layered immune system during hematopoietic development are unclear. Using an irreversible lineage-trac...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.08.013
更新日期:2016-12-01 00:00:00
abstract::The re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural p...
journal_title:Cell stem cell
pub_type: 杂志文章,评审
doi:10.1016/j.stem.2016.11.014
更新日期:2016-12-01 00:00:00
abstract::The current widespread outbreak of Zika virus (ZIKV) infection has been linked to severe clinical birth defects, particularly microcephaly, warranting urgent study of the molecular mechanisms underlying ZIKV pathogenesis. Akt-mTOR signaling is one of the key cellular pathways essential for brain development and autoph...
journal_title:Cell stem cell
pub_type: 杂志文章
doi:10.1016/j.stem.2016.07.019
更新日期:2016-11-03 00:00:00